MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Yang M, Liao LF, Zhang GL, Xiao XL, Lin YW, Nie CM
Article Title: Determination of ATP using a double-receptor sandwich method based on molecularly imprinted membrane and fluorescence-labeled uranyl-salophen complex.
Publication date: 2013
Journal: Analytical and Bioanalytical Chemistry
Volume: 405
Issue: (23)
Page numbers: 7545-7551.
DOI: 10.1007/s00216-013-7217-2

Abstract: A double-receptor sandwich method for the fluorescence determination of adenosine triphosphate (ATP) is proposed in this paper. The solid phase receptor on the surface of glass slides is a molecularly imprinted membrane (MIM) containing an artificial nanocavity. It is constructed by a molecular imprinting technique using adenosine monophosphate (AMP) as a template molecule. The labeled receptor is a uranyl-salophen complex containing a fluorescent group or uranyl-salophen-fluorescein (USF). It is synthesized with salophen, 5-aminofluorescein, and uranyl. In a procedure of determining ATP, ATP in sample solution is first adsorbed on the surface of the glass slide through the combination of the AMP group in ATP with the nanocavity in MIM. Then, the adsorbed ATP binds USF through the coordination reaction of the phosphate group in ATP with uranyl in USF to form a sandwich-type structure of MIM-ATP-USF. The amount of ATP is detected through the fluorescence determination of USF bound on the slide. Under optimal conditions, the linear range for the determination of ATP is 0.3 to 4.8 nmol/mL with a detection limit of 0.041 nmol/mL. The proposed method has been successfully employed for the determination of ATP in real samples with the recoveries of 98.5 to 102.5 %
Template and target information: ATP, adenosine triphosphate
Author keywords: ATP, molecularly imprinted membrane, fluorescence, Uranyl-salophen complex, Double-receptor, Sandwich

  Meitnerium periodic table mug  Scientist peptide mug    mug featuring the element Uranium

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner