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Reference type: Journal
Authors: Rachkov A, McNiven S, Elskaya A, Yano K, Karube I
Article Title: Fluorescence detection of b-estradiol using a molecularly imprinted polymer.
Publication date: 2000
Journal: Analytica Chimica Acta
Volume: 405
Issue: (1-2)
Page numbers: 23-29.
DOI: 10.1016/S0003-2670(99)00743-6
Alternative URL: http://144.206.159.178/FT/38/10159/195942.pdf

Abstract: A fluorescence sensing system has been developed for the determination of β-estradiol using a molecularly imprinted polymer (MIP) and liquid chromatography (LC). Two approaches were explored: (a) direct measurement of the β-estradiol fluorescence; (b) a method based on the competitive displacement by the analyte of a fluorescent compound from specific binding sites in the imprinted polymer. Detection based on the direct mode shows a linear response over the range of 0.1-20 μM (30-5000 ng ml(-1)). The time necessary for the measurement of one sample is about 15 min. This approach makes possible the development of highly selective and sensitive analytical systems based on MIPs which can be prepared with selectivity for a wide variety of substances. (C)2000 Elsevier Science B.V. All rights reserved
Template and target information: β-estradiol


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