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Reference type: Journal
Authors: Rachkov A, Minoura N, Shimizu T
Article Title: Peptide separation using molecularly imprinted polymer prepared by epitope approach.
Publication date: 2001
Journal: Analytical Sciences
Volume: 17
Issue: (Supplement (Proceedings of IUPAC International Congress on Analytical Sciences 2001 (ICAS 2001)))
Page numbers: i609-i612.
Alternative URL: http://wwwsoc.nii.ac.jp/jsac/analsci/ICAS2001/pdfs/0600/0609_1i05n.pdf

Abstract: Peptide separation in the aqueous-rich mobile phase using the molecularly imprinted polymer prepared by the epitope approach as a stationary phase for HPLC was investigated. Usually, for the preparation of stable molecularly imprinted polymers of predetermined ligand selectivity, only relatively low molecular weight compounds are used as templates. According to the early proposed epitope approach, we have synthesized polymers, which are able to recognize the template as well as some larger peptides possessing the same structural fragment as that of the template. After optimization of the composition of chromatographic mobile phase, a clear base-line separation of the equimolar mixtures of template YPLG or oxytocin possessing the same C-terminus sequence as the template, with other peptides was shown. The MIP demonstrated reliable resolution of mixtures containing YPLG up to 75 mM (Rs>1.3), and mixtures containing oxytocin up to 50 mM peptide load (Rs>1.5)
Template and target information: peptide, YPLG, oxytocin, Tyr-Pro-Leu-Gly

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