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Reference type: Journal
Authors: Pluhar B, Ziener U, Mizaikoff B
Article Title: Surface imprinting of pepsin via miniemulsion polymerization.
Publication date: 2013
Journal: Journal of Materials Chemistry B
Volume: 1
Issue: (40)
Page numbers: 5489-5495.
DOI: 10.1039/C3TB20773K
Alternative URL: https://www.researchgate.net/publication/264811227_Surface_imprinting_of_pepsin_via_miniemulsion_polymerization

Abstract: Surface imprinted polymers allow accessibility of the selective binding sites to large molecules such as proteins. In this work, small polymer particles offering a large surface area were prepared via miniemulsion polymerization in the presence of pepsin serving as a template molecule. The influence of four different functional monomers and of the amount of the template on the imprinting effect of pepsin was investigated. After the miniemulsion polymerization and a washing step, stable polymer suspensions with an average particle diameter of 400-600 nm and a specific surface area of 30-65 m2 g-1 were obtained. The results of detailed rebinding experiments revealed that the highest imprinting effect was achieved with (3-acrylamidopropyl)trimethylammonium chloride as a functional monomer and a high amount of the template. These polymer particles also showed selectivity for pepsin against various proteins. This approach provides a fundamental step towards the development of synthetic protein receptors and protein scavenger materials useful in biomimetic assays and for clean-up in biotechnology
Template and target information: protein, pepsin

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