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Abstract: A novel molecularly imprinted polymer (MIP)-based electrochemical sensor was fabricated by depositing carboxyl-functionalized MWNTs (multiwall carbon nanotubes) and electropolymerizing poly-o-phenylenediamine (oPD) in the presence of uric acid (UA) onto a glassy carbon electrode (GCE) surface. An electroactive substance, potassium ferricyanide, was used as the redox probe. Under the optimized conditions, the sensor exhibited good adsorption and high recognition capacity for UA. The MWNTs were treated to obtain carboxylic acid-functionalized MWNTs. Prior to modification, the surface of the bare glassy carbon electrode (GCE) was polished with a 0.3 - and 0.05-μm alumina-water slurry in sequence, and thoroughly ultrasonically rinsed with ethanol and doubly distilled water for 5 min. The response of the imprinted electrode decreased to 9 4% after storing for one week, and 90% of the original responses were retained after two weeks