MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Szultka M, Krzeminski R, Jackowski M, Buszewski B
Article Title: Simultaneous determination of selected chemotherapeutics in human whole blood by molecularly imprinted polymers coated solid phase microextraction fibers and liquid chromatography-tandem mass spectrometry.
Publication date: 2013
Journal: Journal of Chromatography B
Volume: 940
Page numbers: 66-76.
DOI: 10.1016/j.jchromb.2013.09.009
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1570023213004820

Abstract: Development and validation of novel, general liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of amoxicillin (AMOX), cefatoxime (CEF), ciprofloxacin (CIP), daptomycin (DAPTO), fluconazole (FLU), gentamicin (GEN), clindamycin (KLI), linezolid (LIN), metronidazole (MET), moxifloxacin (MOXI) in human whole blood are described. Samples were prepared on solid phase microextraction way with the use of polymeric sorption coatings with molecular imprints and analyzed using a gradient separation over an ACE C18-column (4.6 mm x 150 mm, 3 μm) with isocratic elution. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (0.1% formic acid or 5 mM ammonium acetate) at a flow 0.4 ml/min. The chromatographic run time was kept less than 9 min. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 7.3%, while the accuracy was within 8.4%. The mean recovery of all the analytes ranged from 65.0 to 83.0%. This method was successfully applied to clinical samples from patients with clinically diagnosed bacterial infections process
Template and target information: amoxicillin, AMOX, cefatoxime, CEF, ciprofloxacin, CIP, daptomycin, DAPTO, fluconazole, FLU, gentamicin, GEN, clindamycin, KLI, linezolid, LIN, metronidazole, MET, moxifloxacin, MOXI
Author keywords: Antibiotic drugs, liquid chromatography-mass spectrometry, molecular imprinted polymers, solid phase microextraction, Whole human blood

  Periodic table Vindaloo - the chemical formula for curry shirt  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner