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Abstract: A liquid chromatography-linear ion-trap spectrometry (LC-MS3) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β2-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic hydrolysis by β-glucoronidase/arylsulphatase, and then extracted with perchloric acid. Sample clean-up was performed using β-receptor MIP SPE. A Supelco Ascentis® express Rp-Amide column was used to separate the analytes, and MS3 detection used an electrospray ionisation source in positive-ion mode. Recovery studies were carried out using blank urine samples fortified with the 46 analytes at the levels of 0.5, 1.0 and 2.0 μg l-1. Recoveries were obtained ranging from 60.1% to 109.9% with relative standard deviations (RSDs, n = 7) from 0.5% to 19.4%. The limits of detection (LODs) and limits of quantitation (LOQs) of the 46 analytes in urine were 0.02-0.18 and 0.05-0.60 μg l-1, respectively. As a result of the selective clean-up by MIP SPE and MS3 detection of the target drugs, the sensitivity and accuracy of the present method was high enough for monitoring β2-agonist and β-blocker residues in urine samples. Satisfactory results were obtained in the process of the determination of positive urine samples
Template and target information: β2-agonists, β-blockers, commercial MIP
Author keywords: β2-Agonists, β-Blockers, MIP SPE, LC-MS, urine