Inventors: Yu JH, Zhang CC, Ge SG, Huang JD, Yan M, Wan FW, Ge L, Zhao PN, Gao WQ
Patent Title: Kit for simultaneously detecting and tracing a plurality of environmental endocrine disruptors and application thereof
Publication date: 2010
Publication number: CN101893574

Abstract: The invention relates to the field of environmental endocrine disruptors, and provides a kit for simultaneously detecting a plurality of environmental endocrine disruptors and application thereof. The kit is made by adopting the following steps: respectively preparing potassium permanganate solution, HCL solution and polysorbate-80 solution; uniformly mixing template molecules, a functional monomer, a cross-linking agent, a porogenic agent, an initiator and organic solvent in a certain molar rate to prepare molecularly imprinted sol-gel of the environmental endocrine disruptors; modifying the molecularly imprinted sol-gel on the inner surface of a microporous plate, sealing the inner surface by a preservative film for hours; soaking the modified microporous plate in eluant for eluting; ; and inserting ports of a sample injector of the microporous plate into luminescence reagent solution to obtain the kit for simultaneously detecting a plurality of environmental endocrine disruptors. The kit is connected to a multifunctional analyzer on the microporous plate to detect the environmental endocrine disruptors in an environmental sample extracting solution. The kit has the advantages of simple operation, less dosage of reagent, economy and practicality, low price and accessibility of chemical luminescent reagent, high sensitivity and simple operation.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20101124&DB=EPODOC&locale=en_EP&CC=CN&NR=101893574A&KC=A&ND=4