Inventors: Huang JD, Pei MS, Wang SH, Yu JH, Ge SG, Xing XR, He XR, Lin Q, Zhang XM, Zhu H, Sun NX, Song XY, Yuan L
Patent Title: Preparation method and application of nano-synergistic carbohydrate functionalized molecularly imprinted column for detecting bacterial toxins
Publication date: 2010
Publication number: CN101692046

Abstract: The invention relates to a preparation method of a nano-synergistic carbohydrate functionalized molecularly imprinted column for detecting bacterial toxins, comprising the following steps: selecting a carbohydrate functional monomer which can be used for synthesizing a carbohydrate functionalized molecularly imprinted polymer with the bacterial toxins; preparing solution of the carbohydrate functionalized molecularly imprinted polymer; preparing nano solution; and modifying nano materials and the carbohydrate functionalized molecularly imprinted polymer on the inner surface of a glass tube column. A method for detecting trace bacterial toxins of the invention is characterized by connecting the carbohydrate functionalized molecularly imprinted column prepared by the above method with a chemiluminescence analyzer, respectively pumping chemiluminescence system solution and sample solution into the chemiluminescence analyzer and detecting the bacterial toxins in the samples. The preparation method of the invention has controllability and improves the sensitivity and accuracy of the column. The prepared molecularly imprinted column features strong specificity, high sensitivity and rapid detection speed, can realize high throughout screening of vast samples in short time and lower the detection cost.
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