MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Inventors: Chen DL, Zhang Y, Liu LJ, He LR
Patent Title: Preparation method and application of hybrid peptide with high arginine content in stomach cancer treatment
Publication date: 2018
Publication number: CN107574205A

Abstract: The invention relates to a hybrid peptide with a high arginine content and an application thereof in stomach cancer treatment. The preparation method comprises the following steps: using an ultrahighpressure to preprocess walnut meal and egg-white protein suspension; using alkaline protease and papain to enzymatically hydrolyze the preprocessing product in steps under the assistance of ultrasonicwaves and microwaves; filtering the enzymatic hydrolysate, subjecting obtained supernate to reversed phase high performance liquid chromatography so as to separate target hybrid peptides in the supernate; taking the hybrid peptides as the template molecule, taking acrylic acid and methacrylic acid as the functional monomers, taking triethylene glycol dimethacrylate as the crosslinking agent, taking isopropyl thioxanthone as the initiator, and carrying out polymerization triggered by UV rays to form a surface molecularly imprinted membrane, which is used to separate and enrich target hybrid peptides in the supernate of the enzymatic hydrolysate. The arginine content of obtained hybrid peptide is more than 18%. The MTT method detection result show that the hybrid peptide with a high arginine content can effectively inhibit the proliferation of human stomach cancer SGC-7901 cell. The provided method reduces the product cost and the industrial production is promoted.
URL: https://worldwide.espacenet.com/publicationDetails/biblio?CC=CN&NR=107574205A&KC=A&FT=D&DB=EPODOC&locale=en_EP&date=20180112

  Holmium periodic table mug  Mug featuring the name PeptideChemist spelled out in the single letter amino acid code  I shop perodically customisable tote bag

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner