Abstract: A novel strategy to improve the sensitivity of molecularly imprinted polymer (MIP) sensors based on a double layered (DMIP) hybrid film modified gold electrode was investigated for the determination of testosterone. A conductive poly (anilinomethyltriethoxysilane) film was electrodeposited on the surface of the electrode and a molecularly imprinted polysiloxane thin film was formed on the conductive layer by a sol-gel process to enhance the electrochemical response. The performance of the sensor for testosterone was investigated by square wave voltammetry in the presence of a hexacyanoferrate/ferrite as a redox probe. The prepared sensor provided high recognition for testosterone with a broad linear range from 10 to 100 fM and a low detection limit of 10 fM. Good sensitivity, selectivity, and stability were observed with relative standard deviations less than 5%. Good recovery of testosterone was obtained when the sensor was used for the determination of testosterone in fortified urine
Author keywords: conductive polymer, double layered molecularly imprinted polymer (DMIP), square wave voltammetry (SWV), Testosterone, Voltammetric sensor