Abstract: Aiming at improving the unsatisfactory adsorption and elution efficiency in the conventional magnetic molecularly imprinting technology (MMIT), a reliable strategy to prepare magnetic molecularly imprinted nanoparticles (MMINs) with rapid rebinding and eluting speed was introduced in this work. In order to decrease the mass transfer retention of the templates, glycoprotein immunoglobulin G (IgG) were pre-immobilized to form homogenic and monostratified 3D imprinted cavities on the surface of the magnetic carriers. For the sake of shortening the transfer route of the templates, the polymerizaiton process was precisely controlled to form well-defined molecularly imprinted polymers (MIPs) films with thickness of 12 nm. By this way, the patency of the mass transfer of the tempaltes was maximized, and the length of the transfer route was minimized. As a result, the prepared MMINs showed improved adsorption and elution efficiency compared with the conventional magnetic molecularly imprinted polymers (MMIPs), and exhibited great selectivity towards the templates. Morever, the realization of this stragety provided MMIT with a favorable application foreground in clinical