Abstract: In this paper, mono-disperse well-defined core-shell magnetic molecularly-imprinted polymer (MD-CS-MMIP) was controllably synthesized via modified precipitation polymerization and used as magnetic solid-phase extraction adsorbent for simultaneous determination of six trace mycotoxins in rice samples. The as-prepared MD-CS-MMIP was fully characterized by SEM, TEM, XRD, and TG-DTG. This study is particularly concerned at the influence of oleic acid usage amount for MD-CS-MMIP synthesis on the material morphology, component, and adsorption efficiency. The results revealed that the modification of oleic acid on Fe3O4 surface could availably improve the monodispersion of core-shell MD-CS-MMIP. Moreover, MD-CS-MMIP was used as magnetic solid-phase extraction adsorbent for determination of six mycotoxins residue in rice samples, and the adsorption and desorption conditions of MD-CS-MMIP toward mycotoxins were optimized to obtain the highest adsorption capacity, selectivity, and desorption efficiency. Under the optimum conditions, the limits of quantitation (LOQs) of the developed method toward six mycotoxins were ranging from 0.003 μg kg-1 to 0.01 μg kg-1. The developed method was successfully used for the determination of rice samples with recoveries in range of 86.3-103.1%