Abstract: Hybrid-monomers double templates molecularly imprinted polymers (HDMIPs) were prepared by two methods with in-situ polymerization technique. Hybrid-monomers were prepared with 3-aminopropyltriethoxysilanes (APTES) as an inorganic source and methacrylic acid (MAA)/acrylamide (AM) as an organic source. HDMIPs were used as sorbents for selective isolation and determination theophylline and quercetin in green tea extracts. Fourier transform infrared and field emission scanning electron microscope were used to characterize the morphology and structure information of HDMIPs. The recognition ability and binding capability of HDMIPs for analytes were evaluated by static absorption and Scatchard analysis. The adsorption equilibrium data of two analytes were investigated using three different analysis models: linear, Langmuir and Freundlich. With optimization of solid-phase extraction procedure, a reliable analytical method was developed for highly recognition towards theophylline and quercetin in green tea with satisfactory extraction recoveries of 96.68% and 88.92%. The recoveries of proposed method at three spiked levels analysis were 84.62-96.68% for theophylline and 82.88-88.92% for quercetin. The HDMIPs showed good performance for two targets, and the proposed approach with high affinity of hybrid molecularly imprinted polymers might offer a novel method for the purification of complex samples