Abstract: A class-specific macrolide molecularly imprinted polymer was synthesized by precipitation polymerization using tulathromycin as the template and methacrylic acid as the functional monomer. The polymers revealed different specific adsorption and imprinting factor for macrolides with different spatial arrangement of side chains as well as lactonic ring size. And the molecularly imprinted polymer possessed maximum adsorption capacity (54.1-ámg g-1) and highest imprinting factor (2.4) towards 15-membered ring azithromycin. On the basis of molecularly imprinted polymer dispersive solid-phase extraction, a rapid, selective and reproducible method for simultaneous determination of seven macrolide antibiotics residues in pork was established by using liquid chromatography with tandem mass spectrometry. At spiking levels of 5, 10, 25 and 100 μg kg-1, average recoveries of seven macrolides ranged from 68.6 to 95.5% with intra-day and inter-day relative standard deviations below 8%. The limits of detection and limits of quantification were 0.2-0.5 and 0.5-2.0 μg kg-1, respectively. This article is protected by copyright. All rights reserved
Author keywords: Dispersive solid-phase extraction, Liquid chromatography with tandem mass spectrometry, Macrolide antibiotics, Molecularly imprinted polymers, Pork