Abstract: An accurate, rapid, and sensitive method for the determination of risperidone and 9-hydroxyrisperidone in urine samples was developed by combining water-compatible magnetic molecularly imprinted solid-phase extraction with high performance liquid chromatography. Several variables relating to the efficiency of magnetic solid phase extraction were optimized, including the amount of adsorbent, adsorption time, type of elution solvent, and desorption time. The analytical performance of this method was validated under the optimized conditions. The linearity for risperidone and 9-hydroxyrisperidone was obtained in the range 1-2000 ng mL-1 with correlation coefficient ³ 0.991. Limits of detection of risperidone and 9-hydroxyrisperidone are 0.21 ng mL-1 and 0.24 ng mL-1, respectively. Recoveries at three spike levels (10, 100, and 1000 ng mL-1) ranged from 94.6% to 102.4% with relative standard deviations (%) £ 5.3. These results confirmed that this method can be successfully and facilely used to analyze the multi-residues of risperidone and 9-hydroxyrisperidone in urine samples with high efficiency and good sensitivity
Author keywords: Molecularly imprinted polymer, Water compatibility, Magnetic solid phase extraction, Risperidone, Urine