Abstract: An alumina template modified with ZrO2 was coated with cellulose acetate (CA) containing imprinted molecules for a novel enantioselective membrane. The preparation, morphologies and properties of the different membranes were introduced and investigated. The morphologies of the membranes were examined using a scanning electron microscope. The best performance of CA amounts in casting solution was observed when it was 15 wt.% and choose as the preparation of resulting membrane. S-(+)-mandelic acid was used as imprinted molecules to prepare the molecular imprinted nanochannel membrane which enhanced the permselectivity and flux compared with the traditional membrane and the separation factor was as high as 35. And this membrane has the best separation effect on the (R, S)-mandelic acid when compared with (R, S)-malic acid and (R, S)-lactic acid