Abstract: Herein, a carbon paste electrode, concurrently incorporated with ferrocene and molecularly imprinted polymer nanomaterial (Fc-MIP-CP electrode), is introduced as an innovative sensing platform for detection of thiamine as an example of weak electroactive compounds. The MIP nanoparticles were prepared and embedded with ferrocene together in carbon paste electrode. The prepared electrode was used to detect thiamine via a redox probe (ferrocene) using square wave voltammetry (SWV) technique. Utilizing the electrochemical impedance spectroscopy and cyclic voltammetry results, a new sensing mechanism was proposed based on the MIP gate effect principal. Various parameters, influencing the peak signal (MIP and Fc percentage in carbon paste composite, analysis pH, amplitude and frequency) were optimized. The best results were obtained in analysis pH=9, frequency of 30 Hz and amplitude of 150 mV using 2 and 12 percent of Fc and MIP, respectively. ferrocene signal enhancement with thiamine concentration increasing was linear in range of 1 x 10-6 - 4 x 10-9 M. the detection limit of the sensor was calculated to be 1.0 nM. The selectivity of the proposed electrode was demonstrated by using the analogous molecules and other vitamin B compounds. This method was successfully applied for the determination of thiamine in serum samples and pharmaceuticals