Abstract: A new type of thermo-sensitive dual-template epitope molecular imprinting polymer was prepared coated on magnetic carbon nanotubes (MCNTs@D-EMIP) for simultaneous recognition of human serum albumin (HSA) and transferrin (Trf) via the strategies of dual-template epitope imprinting, metal chelation imprinting and distillation-precipitation polymerization (DPP). C-terminal peptides of HSA and C-terminal peptides of Trf were selected as templates, zinc acrylate and N-isopropylacrylamide (NIPAM) were used as functional monomers, and MCNTs@D-EMIP was prepared by the method of DPP. The two types of template epitopes were immobilized by metal chelation and six-membered ring formed with zinc acylate. MCNTs@D-EMIP was prepared only in 30 min which was much shorter than other polymerization methods. The resultant MCNTs@D-EMIP showed excellent specific recognition ability towards HSA and Trf. The adsorption amounts of MCNTs@D-EMIP for HSA and Trf were 103.67 mg g-1 and 68.48 mg g-1 and imprinting factors were 2.57 and 2.17, respectively. In addition, MCNTs@D-EMIP displayed thermo-sensitive property to realize temperature controlled recognition and release of target proteins. Furthermore, the results of HPLC analysis proved that MCNTs@D-EMIP could be applied to specifically recognize two types of targets simultaneously in bio-sample. The proposed strategy provided a preparation method for thermo-sensitive dual-template epitope imprinting polymer via dual-template imprinting, metal chelation imprinting and DPP