Abstract: Inspired by the recognition processes found in biology such as enzyme-substrate and antibody-antigen interactions, synthetic systems with comparable molecular recognition properties have been investigated during recent years based on molecular imprinting strategies. While materials with recognition capabilities for small molecules (i.e. with low molecular weight) have achieved substantial advancements, the synthesis of molecularly imprinted materials with virus recognition properties remain challenging to date. Likewise, protein-surface and protein-protein interactions are essential for a wide variety of biological applications in biotechnology. In biological sensor technology the coating of surfaces to prevent non-specific adsorption interactions plays an important role. Particularly, polyethylene glycol (PEG) stands out for its high performance in preventing proteins from non-specifically interactions. However, blocking agents like the protein bovine serum albumin (BSA) can also be useful as unspecific binding prevention agent for passivation, without modification of the surface. Herein the influence of BSA as unspecific binding prevention agent is investigated on the enhancements of selectivity from adenovirus-imprinted particles, whereas adenovirus was used for the first time as target species in molecular imprinting. Furthermore, quantitative polymerase chain reaction (qPCR) was used for the first time as virus quantification approach in this context