Abstract: Inspired from the biomimetic polydopamine (pDA)-based self-polymerization technique and Janus nanocomposite structure, an efficient yet simple method of pDA@Au-based Janus-incorporated molecularly imprinted nanocomposite membranes (MINCMs) has been developed. The Janus nanocomposite was obtained by using pDA nanospheres as the supports, and the catechol-reduced Au nanoparticles from Au ions were then grown on the surfaces of pDA nanospheres. Highly regenerative performance and selective separability toward tetracycline (TC) were finally obtained. Because of the formation of this membrane-based Janus nanocomposite surfaces, largely enhanced TC-rebinding capacities (67.43 mg/g), permselectivity (separation factors were all more than 10.5) and rebinding stability (93% of the saturated adsorption capacity after 11 cycling adsorption/desorption cycles) were finally obtained. These results strongly illustrated that incorporation of the Janus nanocomposite into molecularly imprinted membranes would achieve both the high rebinding capacity and the excellent permselectivity. All the synthesis processes were carried out at low temperature and ordinary pressure, which were energy-efficient and environmentally friendly for large-scale applications