Abstract: The synthesis of biomacromolecule imprinted materials is a challenge with the bulkiness and instability of macromolecule, such as protein. A green strategy to prepare hydrophilic protein-imprinted resin (HPIR) was firstly developed using resorcinol and melamine as double functional monomers, hexamethylenetetramine instead of formaldehyde as crosslinker, water as solvent, and bovine serum albumin (BSA) as a dual-function template. BSA as dual-function template could not only act as template but also porogen in the preparation of HPIR. According to the adsorption experiments, the adsorption kinetics of BSA on HPIR obeyed pseudo-second-order adsorption kinetics and the equilibrium data were best described by Langmuir isotherm model. The adsorption capability was 44.3459 mg g-1 (25 °C), and the imprinted factor for binding BSA is 5.78. HPIR showed high adsorption capacity, fast mass transfer rate, and specific selectivity towards BSA in aqueous solvent. More importantly, HPIR can be applied to selectively adsorb target protein BSA in urine without the matrix interference of biological matrix, therefore, it is promising for HPIR to be applied to proteomics, diagnostics, and pharmaceutics
Author keywords: Aqueous molecular imprinting, Hydrophilic resins, Specific Recognition, bovine serum albumin, Biological matrix