Abstract: Magnetic surface molecularly imprinted polymeric microspheres (MMIPs) were prepared by using gallic acid (GA) as segment template for solid extracting ester-catechins as a whole from complex samples. The MMIPs were characterized by Fourier transform infrared spectroscope, transmission electron microscope, and vibrating sample magnetometer. The results of adsorption experiments indicated that the saturated adsorption capacity of GA on MMIPs was 0.21 mmol/g, the imprinting factor was 5.86, and the MMIPs have higher adsorption capacity for ester-catechins than benzoic acid, salicylic acid, and 2,4-dihydroxybenzoic acid. The adsorption process of MMIPs could be better described by pseudo secondary rate equation and be consistent with the Langmuir adsorption model. In addition to GA, the MMIPs could selectively recognize ester-catechins in tea solution without column packing. Thus, MMIPs prepared using GA as segment template could be used in selective separation and enrichment of ester-catechins from tea samples for functional food production