Abstract: Tyramine, an important biogenic amine, occurs in high amounts in certain processed food products that can cause adverse health effects in people. In this study, we developed a simple method to produce a molecularly imprinted fluorescence sensor with high sensitivity and selectivity for tyramine. The reverse microemulsion method was applied to prepare a carrier material, hydrophobic CdSe/ZnS quantum dots (QDs) sensitized with carboxylated graphene (Gra), which was added to the reaction for synthesizing molecularly imprinted polymers (MIPs) using 3-mercaptopropyltriethoxysilane(MPTES) as the functional monomer and tetraethylorthosilicate (TEOS) as the crosslinking agent in the presence of tyramine as the target molecule. The novel sensing material, Gra-QDs@MIPs, was selective for tyramine and generated a concentration-dependent fluorescence signal. Under optimized conditions, the limit of detection was 0.021 mg L-1 and the linear range of detection was 0.07-12 mg L-1. Importantly, we tested the performance of the Gra-QDs@MIPs with real-world samples by demonstrating that it selectively detected around 0.08 or 2 mg L-1 of tyramine in spiked rice wine samples. Hence, the described molecularly imprinted fluorescence sensor for tyramine could serve as a prototype for developing MIP-based assays for food analysis