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Reference type: Journal
Authors: Bartold K, Pietrzyk-Le A, Golebiewska K, Lisowski W, Cauteruccio S, Licandro E, D'Souza F, Kutner W
Article Title: Oligonucleotide Determination via Peptide Nucleic Acid Macromolecular Imprinting in an Electropolymerized CG-rich Artificial Oligomer Analog.
Publication date: 2018
Journal: ACS Applied Materials & Interfaces
DOI: 10.1021/acsami.8b09296

Abstract: We devised and fabricated a chemosensor for determination of genetically relevant 5' GCGGCGGC 3' (G-guanine, C-cytosine) oligonucleotide. For that, we simultaneously electrosynthesized and electrode immobilized a sequence-defined octakis(2,2'-bithien-5-yl) DNA hybridizing probe using both a 'macromolecular imprinting in polymer strategy' and a sequence-programmable peptide nucleic acid (PNA) template. With EIS and SPR transductions under stagnant-solution and FIA, respectively, conditions, we determined the above oligonucleotide with 200 pM EIS limit of detection. With its EIS determined apparent impact factor of ~4.0, the chemosensor was discriminative to both mismatched oligonucleotides and Dulbecco Modified Eagle Medium sample interferences

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