Abstract: In this study, we firstly presented rotational paper-based microfluidic chips (RPADs) combined with MIP technique to detect phenolic pollutants. The proposed rotational paper-based microfluidic chips could implement qualitative and quantitative analysis of two different phenolic contaminants (4-nitrophenol (4-NP) and 2,4,6-trinitrophenol (TNP)) simultaneously. It could be implemented qualitative and quantitative analysis simultaneously through their fluorescence intensity change depending on the structure of quantum dots combined with molecularly imprinted technique. Moreover, the rotational paper-based microfluidic chips gave a low cost, flexible and easy way to operate the entire process conveniently. Under the optimal conditions, the proposed sensors showed high sensitivity and selectivity. Our final experimental results illustrated that the detection limits of 4-NP and TNP in PQ-MIP (Paper-based Quantum dot-MIP) RPADs ranged from 0.5 to 20.0 mg/L, with the detection limits of 0.097 mg/L and 0.071 mg/L, respectively. This novel rotational paper-based microfluidic device shows great potential and versatility to multiplexed, portable and rapid test environmental and biological sample in the future