Abstract: In this work, a kind of molecularly imprinted polymers (MIPs) was prepared by using metronidazole (MNZ) as the template, pyrrole as the functional monomer and the vertically cross-linking two-dimension Sn3O4 (2D Sn3O4) nanoplates as the support. The developed MIP/2D Sn3O4 was characterized by SEM, XRD, XPS and FT-IR. It was modified on a glassy carbon electrode (GCE) to construct a novel MNZ electrochemical sensor. Parameters affecting the performances of the sensor were thoroughly investigated and optimized. The results revealed that 2D Sn3O4 is promising as the support for MNZ imprinting. Owing to the 2 D structure, large active surface, easy functionalization, and high electrical conductivity, MIP/2D Sn3O4 modified electrode showed wide linear range and low detection limit. The differential pulse voltammetric peak currents on the MIP/2D Sn3O4 electrochemical sensor were linear to the concentrations of MNZ in the range of 0.025-2.5 μM. The detection limit of this sensor was down to 0.0032 μM (S/N=3). This MIP/2D Sn3O4 sensor has been successfully used to detect MNZ in honeybees without any separation and accumulation processes, demonstrating that MIP/2D Sn3O4 is one of the promising biomimetic materials for electroanalysis and relevant fields