Abstract: A ternary deep eutectic solvent (TDES) was used as both template and functional monomer in the synthesis of TDES-based molecularly imprinted polymers (TDES-MIPs). A meticulous miniaturized solid-phase microextraction (mini-SPME) method followed by high-performance liquid chromatography (HPLC) were used for the optimal speciation of 3,4-dihydroxybenzoic acid (3,4-DHBA) in the needle of a syringe system with response surface methodology (RSM). Under the optimal conditions for the determination of 3,4-DHBA (amount of adsorbent (2 mg), sample volume (1 mL), cycles for adsorption and desorption (6)), the actual extraction amount was 8.46 μg g-1. The limits of detection (LODs, S/N = 3) for 3,4-DHBA in Ilex chinensis Sims were 0.26-0.31 μg mL-1, and the intra-day and inter-day precision (relative standard deviations, n = 4) after spiking with 5 μg mL-1, 100 μg mL-1, and 200 μg mL-1 were both less than 4.21%. The meticulous method (TDES-MIP-mini-SPME) combined with RSM offers a significant advance over existing methods, because of the meticulous operation and excellent selectivity of 3,4-DHBA from complex samples
Author keywords: Miniaturized solid-phase microextraction, 3,4-Dihydroxybenzoic acid, Ternary deep eutectic solvent-based molecularly imprinted polymers, Response surface methodology, Ilex chinensis Sims