Abstract: This work mainly studied the separation performance of quercetin molecularly imprinted polymer (Qu-MIP) on quercetin (Qu) in the presence of other flavonoids which possessed similar structures partially at least under different variable conditions. Results documented that Qu-MIP exhibited a special selectivity to Qu in all conditions, and the special selectivity would be enlarged obviously when the total concentration and temperature increased. Besides, quantitative analysis of the degree of separation (D) and concentration (C) (or temperature (T)) displayed a well linear relationship between lnD and C (or T), and the intercept of line, which we defined as the Napierian logarithm of eigen separation degree, increased with the increase of molecular size, which meant MIP could separation target compounds from complex solution system well if impurities with similar contracture but larger molecular size; D increased with the increase of concentration and temperature, but the slope showed an inverse variation trend with the size of Xn. Slope almost showed an increase trend with the increase of Xn molecule size. With the elevation of temperature, slope presented a decline trend with the increase of Xn molecule size. Based on the basic principal of physical and chemical, related reasons were analyzed in detail
Author keywords: Qu-MIP, Separation performance, quantitative analysis, Variable conditions