Abstract: A molecularly imprinted membrane (MIM) with teicoplanin (TE) as a template and other related different material membranes (organic nylon microporous membranes, polyvinylidene fluoride (PVDF) membranes, polypropylene membranes) as a support respectively were synthesized for the selective absorption of TE, which can be used for pretreatment biological samples and rapid determination of TE. The results showed that PVDF MIM had the best selectivity of the other three synthesized membranes to be used in the work. Performance of PVDF MIM was examined, including surface structure, adsorption capacity, desorption capacity, selective adsorption capacity, recovery rate, and also the precision, stability, repeatability, linearity and recovery rate of this method were investigated at the same time. Clean-up and enrichment of samples by TE MIM was reliable.capacity, recovery rate, and also the precision, stability, repeatability, linearity and recovery rate of this method were investigated at the same time. Clean-up and enrichment of samples by TE MIM was reliable. The results showed that PVDF MIM had the best selectivity of the other three synthesized membranes to be used in the work. Performance of PVDF MIM was examined, including surface structure, adsorption capacity, desorption capacity, selective adsorption capacity, recovery rate, and also the precision, stability, repeatability, linearity and recovery rate of this method were investigated at the same time. Clean-up and enrichment of samples by TE MIM was reliable