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Reference type: Journal
Authors: Liu YJ, Wang YZ, Dai QZ, Zhou YG
Article Title: Magnetic deep eutectic solvents molecularly imprinted polymers for the selective recognition and separation of protein.
Publication date: 2016
Journal: Analytica Chimica Acta
Volume: 936
Page numbers: 168-178.
DOI: 10.1016/j.aca.2016.07.003
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0003267016308248

Abstract: A novel and facile magnetic deep eutectic solvents (DES) molecularly imprinted polymers (MIPs) for the selective recognition and separation of Bovine hemoglobin (BHb) was prepared. The new-type DES was adopted as the functional monomer which would bring molecular imprinted technology to a new direction. The amounts of DES were optimized. The obtained magnetic DES-MIPs were characterized with fourier transform infrared spectrometry (FT-IR), thermogravimetric analysis (TGA), field emission scanning electron microscope (FESEM), dynamic light scattering (DLS), elemental analysis and vibrating sample magnetometer (VSM). The results suggested that the imprinted polymers were successfully formed and possessed a charming magnetism. The maximum adsorption capability (Qmax) and dissociation constant (KL) were analyzed by Langmuir isotherms (R2 = 0.9983) and the value were estimated to be 175.44 mg/g and 0.035 mg/mL for the imprinted particles. And the imprinted particles showed a high imprinting factor of 4.77. In addition, the magnetic DES-MIPs presented outstanding recognition specificity and selectivity so that it can be utilized to separate template protein from the mixture of proteins and real samples. Last but not least, the combination of deep eutectic solvents and molecular imprinted technology in this paper provides a new perspective for the recognition and separation of proteins
Template and target information: protein, bovine hemoglobin, BHb
Author keywords: Magnetic, molecular imprinted polymers, Deep eutectic solvents, Recognition and separation, protein

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