Reference type: Journal
Authors: Hashemi SH, Kaykhaii M, Keikha AJ, Sajjadi Z
Article Title: Application of Box-Behnken design in response surface methodology for the molecularly imprinted polymer pipette-tip solid phase extraction of methyl red from seawater samples and its determination by spectrophotometery.
Publication date: 2018
Journal: Marine Pollution Bulletin
Volume: 137
Page numbers: 306-314.
DOI: 10.1016/j.marpolbul.2018.10.037
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0025326X18307471

Abstract: In this paper, a rapid, selective and effective technique, pipette-tip solid phase extraction based on molecularly imprinted polymer, was used for extraction and pre-concentration of methyl red (MR) prior to its determination by spectrophotometer. Variables influencing extraction efficiency including type and volume of eluent solvent, sample volume, number of cycles of extraction and elution, amount of sorbent and pH of the sample solution were optimized with two methods of one-variable-at-a-time and response surface methodology (RSM). For RSM optimization, seven factors in three-levels were utilized for Box-Behnken experimental design. Under optimum conditions, a linear calibration graph in the range of 3.0-300.0 μg L-1 for MR was resulted. The limit of detection of proposed method was 0.50 μg L-1 for MR. Finally, the investigated method was used for the determination of MR in seawater and the mean recoveries were calculated to be 84.0-98.0% with mean RSD of 2.5-6.7%
Template and target information: methyl red, MR
Author keywords: Methyl red, Pipette-tip solid phase extraction, molecularly imprinted polymer, Box-Behnken experimental design, Response surface methodology, Seawater analysis