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Abstract: Food-borne biotoxins have become one of the most significant concerns of food-safety; therefore, both the in vitro determination and in vivo regulation of the food-borne biotoxins are of great importance. In the present study, a persistent luminescence nanophosphor-involved molecularly imprinted nanoplatform (PLNP@MIP) was well-constructed for both the in vitro and in vivo investigations of the food-borne biotoxins. This PLNP@MIP core-shell structured nanoplatform demonstrated the in vitro recognition performance and in vivo tracing function to the respective target biotoxin, integrating the specific matching and binding capability of MIP and autofluorescence-free bioimaging ability of PLNPs. The triple-doped zinc gallogermanate (ZGGO) nanophosphors were introduced as the emission core, showing super-long afterglow, excellent stability and biocompatibility, low toxicity, and intense NIR luminescence. The MIP shell coating onto the PLNPs was performed via precipitation polymerization using methacrylic acid (MAA) as a monomer, ethylene glycol dimethacrylate (EDMA) as a cross-linker, and 2,2'-azobis(2-methylpropionitrile) (AIBN) as an evocating agent, which was universal for three target biotoxins. The well-characterized PLNP@MIP possessed a typical core-shell structure, relatively uniform nanosize, highly selective recognition capability, and adsorption capacity. With the assistance of background-free in vivo persistent luminescence imaging and NIR-LED re-activability, the imprinting nanoplatform not only could realize the highly sensitive and selective determination of target biotoxins by MIP-SPE preconcentration from specific samples, but also demonstrated great potential for the real-time tracing of the target biotoxin inside a living body, so as to provide more accurate and in situ information about the hazardous mechanisms and damaging behaviors of biotoxins that originated from food
Template and target information: biotoxins