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Abstract: Cross-linked enzyme aggregates (CLEAs) is a novel kind of carrier-free immobilized enzyme. It has many advantages including simple preparation procedure, high catalytic activity, easy recovery and increased stability. In recent years, a series of new progress has been made in preparation of CLEAs through combining material science, molecular imprinting engineering, medium engineering and reaction engineering with enzyme immobilization. Carrier-immobilized CLEAs, encapsulated CLEAs, imprinted CLEAs, combi-CLEAs and CLEAs membrane slurry reactor are respectively discussed,and their applications in chiral separation and antibiotics production are introduced. Important factors influencing the activity of CLEAs are analyzed and most recent developments of CLEAs are summarized in this paper. In addition, some prospects for this attractive technology are also presented. It will be helpful for relevant researches in bioengineering, enzyme technology, chemical engineering and materials science
Template and target information: Review - cross-linked enzyme aggregates
Author keywords: cross-linked enzyme aggregates (CLEAs), enzyme immobilization, biocatalysis and biotransformation