Inventors: Zhang DB
, Wo AB
, Qu GR
Method for directly purifying tetracycline in water-containing sample by using molecularly imprinted polymer
Publication date: 2009
The invention relates to a method for directly purifying tetracycline in samples containing water by utilizing molecular imprinted polymer in the technical field of biotechnology. The steps are: the template molecule of tetracycline, the functional monomer of methacrylic acid, mediated imprinted metal ions and the crosslinker of ethylene glycol dimethacrylate are mixed and dissolved in a pore-generating agent, and an evocating agent is added, the mouth is sealed, after the compositive polymer is mashed, the minute grains are removed, the template molecule is removed by extraction, the polymer in which the template molecule is removed is dried and stays overnight, and the molecular imprinted polymer of tetracycline is obtained; the molecular imprinted polymer of tetracycline is filled in a solid-phase extraction pillar canal; the solid-phase extraction pillar canal is activated and pre-treated, a urine sample treated by KH2PO4 is added, and the tetracycline is leached and purified through the solid-phase extraction pillar canal. The method is characterized by high selection, high specificity, harmful environment resistance and the direct application in water environment.
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