Abstract: DNA-imprinted gels were prepared by molecular imprinting using two ligand ssDNAs for a target DNA. After 3'acryloyl- and 5'acryloyl-ssDNAs were hybridized with a target DNA as a template, they were copolymerized with acrylamide and N,N'-methylenebisacrylamide by redox copolymerization. Then the DNA-imprinted gels were obtained by removing the template DNA from the resultant networks. The nonimprinted gels were also prepared by the same way without the template DNA. The swelling behavior of the DNA-imprinted gels, nonimprinted gels and PAAm gel in response to the target DNA or its analogues was investigated to evaluate recognition ability of DNA-imprinted gels
Template and target information: DNA
Author keywords: DNA, gels, molecular imprinting, SNPs detection, Stimuli-responsive gels

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