Abstract: Objective To investigate the optimal condition for complete removal of the template molecules from vinblastine(VLB)-imprinted polymer.Method The prepared polymers were packed into the cartridges of solid-phase extraction column and washed by methanol-glacial acetic acid mixture with different proportions.The contents and recoveries of VLB in the effluents were determined.Results Polymer extraction with methanol-glacial acetic acid(9:1,V/V)resulted in VLB recovery of 91.73%,but template bleeding was observed because of incomplete VLB removal.Using methanol-glacial acetic acid(6:4,V/V)as the extraction solvent,the recovery of VLB reached 98.03% with less solvents and extract times.The polymers could selectively adsorb VLB through non-covalent interactions and still exhibited strong affinity for the template molecule but not for the structural analogue vincristine after extraction with methanol-glacial acetic acid(6:4,V/V).Conclusion Methanol-glacial acetic acid(6:4,V/V)is an ideal extract solvent for complete template molecule removal from the polymers,and the processed polymers possess stable capacity of specific recognition and selectivity to the template.
Template and target information: vinblastine, VLB
Author keywords: molecularly imprinted polymer, Vinblastine, removal, template, recovery

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