Abstract: Temperature-sensitive molecularly imprinted microgels (MIGs) exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate (PAD-His) as the functional macromonomer and p-nitrophenyl phosphate (NPP) as the stable transition state analogue (TSA) as well as Co2+ as the coordination center. The catalytic activity of MIGs was greatly influenced by the amount of the template, and could be modulated by temperature. The hydrolysis kinetics of p-nitrophenyl acetate (NPA) in the presence of MIGs could be described by the Michaelis-Menten equation. The Michaelis-Menten constant and maximum velocity were found to be 2.2 x 10 -5 mol/L and 2.04 x 10 8 mol/h, respectively. In addition, the MIGs were found to have a high catalytic selectivity to NPA
Template and target information: p-nitrophenyl phosphate, NPP, transition state analogue, TSA, p-nitrophenyl acetate, NPA
Author keywords: molecular imprinting, microgels, esterase, temperature sensitivity, dextran

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