Abstract: Objective:To prepare a baicalein molecularly imprinted monolithic column and study its specific recognition ability.Methods:A baicalein molecularly imprinted monolithic column was prepared by in-situ polymerization using methacrylic acid(MAA) as a functional monomer,ethylene glycol dimethacrylate(EDMA) as a crosslinking agent,the mixture of dimethylformamide(DMF) and dodecanol as porogenic reagent,and 2,2'-azobisisobutyronitrile(AIBN) as an initiator.The specific recognition ability for baicalein and wogonin was studied in different mobile phase.Effects of flow rate and temperature on retention and separation factor were investigated.Results:The best ratio of template and functional monomer was 1:6.The baicalein molecularly imprinted monolithic polymer has specific recognition ability for the baicalein molecule.Baicalein and wogonin were separated successfully.Conclusion:The prepared molecularly imprinted monolithic column can be used as the separation media of baicalein with high affinity and good specific recognition ability.
Template and target information: baicalein
Author keywords: molecular imprinting, monolithic column, baicalein, wogonin, in-situ polymerization

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