Abstract: A new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was developed, characterized, and used for direct analysis of chlorpromazine in human plasma samples. The RAMIP-BSA was synthesized using chlorpromazine, methacrylic acid, and ethylene glycol dimethacrylate as template, functional monomer, and cross-linker, respectively. Glycerol dimethacrylate and hydroxy methyl methacrylate were used to promote a hydrophilic surface (high density of hydroxyl groups). Afterward, the polymer was coated with BSA using glutaraldehyde as cross-linker, resulting in a protein chemical shield around it. The material was able to eliminate ca. 99 % of protein when a 44-mg mL-1 BSA aqueous solution was passed through it. The RAMIP-BSA was packed in a column and used for direct analysis of chlorpromazine in human plasma samples in an online column switching high-performance liquid chromatography system. The analytical calibration curve was prepared in a pool of human plasma samples with chlorpromazine concentrations ranging from 30 to 350 μg L-1. The correlation coefficient obtained was 0.995 and the limit of quantification was 30 μg L-1. Intra-day and inter-day precision and accuracy presented variation coefficients and relative errors lower than 15 % and within -15 and 15 %, respectively. The sample throughput was 3 h-1 (sample preparation and chromatographic analysis steps) and the same RAMIP-BSA column was efficiently used for about 90 cycles
Template and target information: chlorpromazine
Author keywords: molecularly imprinted polymer, Restricted access material, BSA layer, RAMIP-BSA, human plasma, Chlorpromazine

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