Abstract: One of the most toxic and hazardous elements affecting human health is lead which is known as a carcinogenic factor, causing harmful effects on human metabolic procedure. The present study deals with the preparation of a novel and selective magnetic ion-imprinted polymer (Fe3O4@SiO2@IIP) with the application of isatin as a ligand and 4-vinilpyridine (4-VP) as a functional monomer for the selective and efficient extraction of Pb(II) ions from various fruits and vegetable samples. A Box-Behnken design was applied with several variables for optimization of the extraction and elution steps. In the selected conditions, the limit of detection, preconcentration factor, and sorption capacity of this new polymer were 0.13 ng mL-1, 324.3, and 83 mg g-1, respectively. Finally, the feasibility of the Fe3O4@SiO2@IIP nanoparticles was evaluated by extraction and determination of Pb(II) ions in different fruits and vegetables including (apple, kiwi, carrot, cantaloupe, peach, cucumber, and tomato)