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Reference type: Journal
Authors: Baydemir G, Denizli A
Article Title: Heparin removal from human plasma using molecular imprinted cryogels.
Publication date: 2015
Journal: Artificial Cells, Nanomedicine, and Biotechnology
Page numbers: 1-10.
DOI: 10.3109/21691401.2014.897631

Abstract: In this study, heparin-imprinted poly(hydroxyethyl methacrylate-N-[(3-dimethylamino)-propyl] methacrylamide) cryogel column (HpMIP) was synthesized for removal of Hp from human plasma using molecular imprinting technique. Hp removal studies were performed from both aqueous solution and human plasma. Selectivity studies were performed using fast protein liquid chromatography (FPLC) system. The obtained results showed that the HpMIP column can remove Hp from both aqueous solutions and human plasma samples selectively. Ninety per cent of Hp was removed from 28 U/mL of human plasma samples successfully. Non-imprinted cryogel column (NIP) and plane PHEMA column were also synthesized to compare selectivity and non-specific adsorption properties
Author keywords: cryogels, Heparin removal, Molecularly imprinted polymers, PHEMA


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