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Reference type: Journal
Authors: Göktürk I, Üzek R, Uzun L, Denizli A
Article Title: Synthesis of a specific monolithic column with artificial recognition sites for L-glutamic acid via cryo-crosslinking of imprinted nanoparticles.
Publication date: 2015
Journal: Artificial Cells, Nanomedicine, and Biotechnology
Page numbers: 1-8.
DOI: 10.3109/21691401.2015.1011806

Abstract: In this study, a new molecular imprinting (MIP)-based monolithic cryogel column was prepared using chemically crosslinked molecularly imprinted nanoparticles, to achieve a simplified chromatographic separation (SPE) for a model compound, L-glutamic acid (L-Glu). Cryogelation through crosslinking of imprinted nanoparticles forms stable monolithic cryogel columns. This technique reduces the leakage of nanoparticles and increases the surface area, while protecting the structural features of the cryogel for stable and efficient recognition of the template molecule. A non-imprinted monolithic cryogel column (NIP) was also prepared, using non-imprinted nanoparticles produced without the addition of L-Glu during polymerization. The molecularly imprinted monolithic cryogel column (MIP) indicates apparent recognition selectivity and a good adsorption capacity compared to the NIP. Also, we have achieved a significant increase in the adsorption capacity, using the advantage of high surface area of the nanoparticles
Author keywords: aminoethyl methacrylate nanoparticle, cryo-crosslinking, Cryogel, glutaraldehyde, L-glutamic acid, Molecular imprinting

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