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Reference type: Journal
Authors: Vlakh EG, Stepanova MA, Pisarev OA, Tennikova TB
Article Title: Preparation and characterization of macroporous monoliths imprinted with erythromycin.
Publication date: 2015
Journal: Journal of Separation Science
Page numbers: n/a.
DOI: 10.1002/jssc.201500438

Abstract: The synthesis of macroporous molecularly imprinted monoliths was performed using the monomers system 2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate and erythromycin as a template. The copolymerization was carried out in situ inside 50 mm x 4.6 mm i.d. stainless-steel tubing. The morphology of the monoliths was examined with scanning electron microscopy. The porous characteristics were determined both from the data of hydrodynamic permeability of monoliths and by means of mercury intrusion porosimetry. The retention parameters of target substance (erythromycin), values of calculated imprinting factors and apparent dynamic dissociation constants were obtained for monoliths prepared with the application of different amount of template (4, 8 and 12 mol%). The separations of the mixtures azithromycin/erythromycin and ciprofloxacin/erythromycin were demonstrated. Additionally, the possibility of erythromycin quantification in human blood plasma was shown. This article is protected by copyright. All rights reserved
Author keywords: antibiotics, Artificial Receptors, high-performance liquid chromatography, Macroporous polymer monoliths, Molecular imprinting


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