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Reference type: Journal
Authors: Jiang H, Jiang DL, Shao JD, Sun XL
Article Title: Magnetic molecularly imprinted polymer nanoparticles based electrochemical sensor for the measurement of Gram-negative bacterial quorum signaling molecules (N-acyl-homoserine-lactones).
Publication date: 2015
Journal: Biosensors and Bioelectronics
DOI: 10.1016/j.bios.2015.07.045
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566315302943

Abstract: We have developed a novel and economical electrochemical sensor to measure Gram-negative bacterial quorum signaling molecules (AHLs) using magnetic nanoparticles and molecularly imprinted polymer (MIP) technology. Magnetic molecularly imprinted polymers (MMIPs) capable of selectively absorbing AHLs were successfully synthesized by surface polymerization. The particles were deposited onto a magnetic carbon paste electrode (MGCE) surface, and characterized by electrochemical measurements. Differential Pulse Voltammetry (DPV) was utilized to record the oxidative current signal that is characteristic of AHL. The detection limit of this assay was determined to be 8 x 10-10 mol L-1 with a linear detection range of 2.5 x 10-9 mol L-1 to 1.0 x 10-7 mol L-1. This Fe3O4@SiO2-MIP-based electrochemical sensor is a valuable new tool that allows quantitative measurement of Gram-negative bacterial quorum signaling molecules. It has potential applications in the fields of clinical diagnosis or food analysis with real-time detection capability, high specificity, excellent reproducibility, and good stability
Author keywords: N-acyl-L-homoserine lactones, Magnetic molecularly imprinted polymers, electrochemical sensor, Fe3O4


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