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Reference type: Journal
Authors: Lei Y, Xu GH, Wei FD, Yang J, Hu Q
Article Title: Preparation of a stir bar coated with molecularly imprinted polymer and its application in analysis of dopamine in urine.
Publication date: 2014
Journal: Journal of Pharmaceutical and Biomedical Analysis
Volume: 94
Page numbers: 118-124.
DOI: 10.1016/j.jpba.2014.01.041
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0731708514000788

Abstract: A molecularly imprinted polymer coated stir bar (MIP-SB) using dopamine as a template was fabricated. The adsorptive capacity of the MIP-SB was almost 4 times over that of non-imprinted stir bar. The MIP-SB showed good extracting selectivity for dopamine when used to adsorb dopamine and its analogs. An analytical method to determine dopamine in urine sample was established by combining MIP-SB sorptive extraction with HPLC-fluorescence detector. The linear range of dopamine concentration was 0.378-18.9 ng/ml with correlation coefficient of 0.9990, and the limit of quantification was about 0.0945 ng/ml (S/N = 10). The recoveries of dopamine with spiked urine samples at three different levels were between 92.3 and 106.9%, and the relative standard deviations were within 13.2% (n = 3). The method is simple and suitable for the determination of dopamine in human urine for clinical application
Template and target information: dopamine
Author keywords: molecularly imprinted polymer, Stir bar sorptive extraction, dopamine, human urine


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