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Abstract: Molecularly imprinting technique can be used to create polymeric materials with recognition sites providing high selectivity and affinity towards template molecules. This technique was successfully applied for the MIP synthesis via thermal polymerization method at 60 °C by using methacrylic acid (MAA) as a functional monomer. In this study, we prepared molecularly imprinted polymers with different amounts of template, porogen and cross-linker for the purpose of optimizing the binding capacity towards γ-oryzanol. MINITAB 15 was employed for the analysis of variance (ANOVA) and the regression model. The polynomial regression model showed 95.3% accuracy for estimating the adsorption capacity of MIP. The analysis of variance with 95% confidence level suggested significant interaction between the amount of template and the amount of cross-linker on the adsorption capacity of MIPs. The maximum adsorption capacity of MIPs was 1.728 mg/g-1 adsorbent. The synthesized MIP was reused five times without significant losses of performance. The adsorption isotherm can be described by Freundlich isothermal model
Template and target information: γ-oryzanol
Author keywords: Gamma-oryzanol, molecular imprinted polymer (MIP), Analysis of variance (ANOVA), molecular recognition, methacrylic acid (MAA)