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Reference type: Journal
Authors: Li XJ, Zhang BL, Tian L, Li W, Xin TJ, Zhang HP, Zhang QY
Article Title: Effect of carboxyl density at the core-shell interface of surface-imprinted magnetic trilayer microspheres on recognition properties of proteins.
Publication date: 2014
Journal: Sensors and Actuators B: Chemical
Volume: 196
Page numbers: 265-271.
DOI: 10.1016/j.snb.2014.01.109
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400514001348

Abstract: A novel protein surface-imprinted magnetic trilayer composite microsphere (Fe3O4@HEA@protein-MIPs) was designed and prepared. The objective of this study was to study the effect of carboxyl density at the core-shell interface on recognition performance. The imprinting process was carried out on the surface of Fe3O4@hydroxyethyl acrylate (Fe3O4@HEA) microsphere modified by maleic anhydride (MAH) in the presence of functional monomer acrylamide (AM) and cross-linker N,N'-methylenebisacrylamide (MBA). The results revealed that the adsorption rate, adsorption capacity and recognition capacity of surface-imprinted magnetic microsphere increased with increasing carboxyl density. Meanwhile, the increase of carboxyl density was conducive to improve the imprinting efficiency of the proteins with high isoelectric point (pI > 7). Furthermore, the effect of carboxyl at the core-shell interface on imprinting efficiency was restricted by the thickness of imprinted layer, and the critical thickness which related to the size of template protein was obtained by experiments
Template and target information: protein, BSA, bovine serum albumin
Author keywords: surface imprinting, Carboxyl density, protein recognition, Magnetic microspheres

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