MIPs logo MIPdatabase     MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Guo HB, Liu YH, Fang BH, Ding HZ, Wang MY, He LM
Article Title: Determination of valnemulin residues in porcine tissues by molecularly imprinted solid-phase extraction coupling with high-performance liquid chromatography.
Publication date: 2014
Journal: Journal of Liquid Chromatography & Related Technologies
Volume: 37
Issue: (11)
Page numbers: 1597-1613.
DOI: 10.1080/10826076.2013.803203

Abstract: A specific and simple high-performance liquid chromatography (HPLC) method for molecularly imprinted solid-phase extraction of the valnemulin from porcine tissues samples was investigated. Molecularly imprinted polymers (MIPs) with high selectivity to valnemulin were synthesized using pre-valnemulin as a dummy template. Valnemulin was extracted with acetonitrile from porcine tissues. Analysis was performed on a C18 column by detection in 210 nm wavelength. The isocratic elution program was 65%acetonitrile in phosphate buffer solution (pH 2.5) with a flow rate of 1.0mL/min. The correlation coefficient (r) for calibration curve is higher than 0.999 within the experimental concentration range. Under the optimum conditions, the polymer sorbent can selectively extract and enrich valnemulin from porcine muscle, lung, and kidney tissues. Mean recoveries of valnemulin in porcine tissues spiked at 0.50, 5.0, and 50mg/kg ranged from 80.5% to 94.8%, with intra-day and inter-day relative standard deviations less than 10%. The limit of detection was approximately 0.1mg/kg and the limit of quantification was 0.25mg/kg. The MIP selectivity was evaluated by checking three drugs with similar and different structures to that of pre-valnemulin. The imprinted cartridge was better than the non-imprinted, C18 and MCX cartridges in terms of both recovery and selectivity
Template and target information: valnemulin
Author keywords: HPLC, Molecularly imprinted polymers, porcine tissues, Solid-phase extraction, UV, Valnemulin


  SMI logo mouse mat  His Periodic table keyring  Lab Chick script shirt

Molecules Special Issue call     Sensors Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon