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Reference type: Journal
Authors: Uygun M, Karagözler AA, Denizli A
Article Title: Molecularly imprinted cryogels for carbonic anhydrase purification from bovine erythrocyte.
Publication date: 2014
Journal: Artificial Cells, Nanomedicine, and Biotechnology
Volume: 42
Issue: (2)
Page numbers: 128-137.
DOI: 10.3109/21691401.2013.864663

Abstract: Molecularly imprinted PHEMAH cryogels were synthesized and used for purification of carbonic anhydrase from bovine erythrocyte. Cryogels were prepared with free radical cryopolymerization of 2-hydroxyethyl methacrylate and methacryloylamido histidine and characterized by swelling degree, macroporosity, FTIR, SEM, surface area and elemental analysis. Maximum carbonic anhydrase adsorption of molecularly imprinted PHEMAH cryogel was found to be 3.16 mg/g. Selectivity of the molecularly imprinted cryogel was investigated using albumin, hemoglobin, IgG, γ-globulin, and lysozyme as competitor proteins and selectivity ratios were found to be 15.26, 60.05, 21.88, 17.61, and 17.42, respectively. Carbonic anhydrase purity was demonstrated by SDS-PAGE and zymogram results
Template and target information: protein, carbonic anhydrase
Author keywords: carbonic anhydrase, Cryogel, molecularly imprinted polymer, purification


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